Preparation CLO Test (Campylobacter Like Organism ) for Detection of Helicobactor Pylori

Abstract

Helicobacter pylori infection is the most important reason of chronic gastritis. H. pylori is a spiral shaped, gram-negative bacterium that colonizes the human stomach. Campylobacter like organism (CLO Test) for detecting Helicobacter pylori infection in the stomach is based on the activity of urease enzyme in a biopsy specimen. The biopsy specimen was taken from the stomach (antrum and /or corpus) and examined immediately. The development of the color in the test gel informs whether urease enzyme is present in the biopsy sample or not. In strong H.pylori colonization (high density) the reaction time is (2 min), in low density H. pylori colonization the reaction can take up to (30 min). H. pylori producesed large amount of urease enzyme which degrades urea to ammonia (NH3). The formed ammonia is detected by an indicator color present in the gel.