Extraction and Purification of Ellagic Acid from White Flash Pomegranate and Study its Anticancer Activity in Vitro and Bioavailability. - استخلاص وتنقية حامض الايلاجيك من شحم الرمان ودراسة فعاليته على بعض الخلايا السرطانية ودراسة التوافر الحيوي

Abstract

Ellagic acid (EA) and hydrolysable ellagitannins (ETs) were extracted from white flesh pomegranate ; the ellagic acid was implicated with potent antioxidant , anticancer and antiatherosclerotic biological properties. Ellagic acid act as excellent scavenger for chemical causing cancer, it form a layer on (DNA) to prevent free radicals damaging. The concentration –time curve were constructed after administration of (80 mg) ellagic acid capsules to (20) healthy volunteers (14 male , 6 females). The calibration curve for quantification of (EA) was linear (r2 (Correlation Coefficient) = 0.9998) over concentration range from (400 to 6.25) ng/ml. The maximum concentration of ellagic acid (C max) was achieved in plasma of volunteers after[1 h (T max)]. The mean serum elimination half life was about (6.21 ± 1.35) h . The recovery of ellagic acid in plasma at different concentration from (50 - 400) ng/ml were between (101-117) % .