Delignification and Bioethanol Production from Different Local Straws and Brans by Enzymes Combinations/ازالة اللكنين وانتاج الايثانول الحيوي من مختلف انواع القش والنخالة بواسطة انزيمات مقترنة

Abstract

In this study, the digestion of different local straws and brans by three enzymes combinations (Lactase, Xylanase and Cellulase ) simultaneously or sequentially had been investigated. The catalytic reaction was carried out in the presence of synthetic and natural mediators (HBT and Vanillin) with different enzymes doses at different (pH) values . Delignification before and after enzymatic treatment and reducing sugars after treatment had been estimated. The higher delignification of (12.5%) and (1.24 mg /ml) reducing sugars obtained from ( 75 mg/ml) wheat bran (1.65 %) transformation after treated with (10) U of LactaseLactase , Xylanase and Cellulase in the presence of (8mM) HBT and (2mM) Vanillin at different )pH( and temperature .